#Minitube cryoguard media androhep plus#

Samples were aliquoted in cryopreservation extender F1 or F2. Ejaculates were collected, diluted (1:1), and cooled before shipping at 17 ☌ overnight.

#Minitube cryoguard media androhep plus#

N2 - This study was conducted to evaluate whether there were differences in viability of cryopreserved semen when using two different freezing (Minitube Cryoguard – F1 or Androstar® CryoPlus – F2) and thawing (Minitube Cryoguard Thawing solution – T1 or Androstar® Plus – T2) extenders. The authors thank: São Paulo Research Foundation, Brazil (FAPESP BEPE – Research Internship Abroad Scholarships Grant 2019/15668–3 - AFCA ) and National Council for Scientific and Technological Development (CNPq) grant number 308989/2020–1 (AFCA). T1 - In vitro effects of two different commercial freezing and thawing extenders on boar sperm quality It is concluded different combinations of commercial freezing extenders and thawing solutions have effects on the quality of cryopreserved boar semen in vitro.', The use of T2, as compared with T1 thawing extender, resulted in an enhanced integrity of the plasma and acrosomal membranes (P = 0.008). The sperm plasma and acrosomal membrane integrity (AIMI) were greater (P = 0.009) when samples were preserved in F1 compared to F2 extender. Sperm thawed in T1 had a greater TMOT (P = 0.008) and PMOT (P = 0.033) at all times evaluated. The sperm progressive motility (PMOT) as time post-thawing increased was greater (P = 0.015) when dilutions occurred using F1 compared with F2 extender. There was no interaction between F × T × Time (P > 0.05), and no interaction between F × T (P > 0.05). The integrity assessments of the plasma and acrosomal membranes were performed at 30 and 360 min after thawing. The sperm in diluted semen were evaluated for motility kinetics at 30, 180, and 360 min after thawing. Four straws from each treatment sample were thawed and diluted in T1 or T2, resulting in four treatments (F1-T1, F1-T2, F2-T1, and F2-T2). It is concluded different combinations of commercial freezing extenders and thawing solutions have effects on the quality of cryopreserved boar semen in vitro.Ībstract = 'This study was conducted to evaluate whether there were differences in viability of cryopreserved semen when using two different freezing (Minitube Cryoguard – F1 or Androstar Plus – T2) extenders.

This study was conducted to evaluate whether there were differences in viability of cryopreserved semen when using two different freezing (Minitube Cryoguard – F1 or Androstar® CryoPlus – F2) and thawing (Minitube Cryoguard Thawing solution – T1 or Androstar® Plus – T2) extenders.